Two distinct polynucleotide ligases from rat liver
نویسندگان
چکیده
منابع مشابه
Two distinct conformations of rat liver ribosomal 5S RNA.
Three different conformers of rat liver 5S ribosomal RNA were investigated by partial nuclease cleavage technique using S1 nuclease and cobra venom endoribonuclease (CVE) as conformational probes. Urea-treated and renatured 5S RNA co-migrate on non-denaturing gels, but exhibit distinct differences in their nuclease cleavage patterns. The most prominent differences in S1 nuclease and CVE accessi...
متن کاملPartial purification and properties of rat liver mitochondrial polynucleotide phosphorylase.
1. Polynucleotide phosphorylase was partially purified from the inner membrane of rat liver mitochondria. 2. The partially purified particulate enzyme catalyses phosphorolysis of poly(A), poly(C), poly(U) and RNA to nucleoside diphosphates. 3. It is devoid of nucleoside diphosphate-polymerization activity. 4. Variable amounts of ADP/P(i)-exchange activity are associated with the polynucleotide ...
متن کاملIsolation of Two Distinct Classes of Polysomes from a Nuclear Fraction of Rat Liver
Isolated rat liver nuclei were washed with Triton-X-100 in the presence of liver cell sap. This treatment liberated a fraction of polysomes which were isolated by differential centrifugation and were designated "outer membrane polysomes." The outer membrane polysomes synthesized protein in vivo. Shortly after injection of orotic acid-(14)C, the RNA of outer membrane polysomes had a higher speci...
متن کاملStudies on polynucleotide-stimulated amino acyl transfer from soluble-RNA to rat liver ribosomes.
Recent evidence has provided experimental support for the concept of a "messenger RNA" concerned with the transmission of information from DNA to protein. -5 The demonstrations that biosynthetically prepared polyribonucleotides of varying base compositions stimulate the incorporation of individual amino acids in an amino acid-incorporating system from E. coli6 -' suggest that such polymers act ...
متن کاملMacromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.
In the presence of high concentrations of any of several types of macromolecules, DNA ligase preparations from rat liver nuclei or from Escherichia coli actively catalyze the blunt-end ligation of DNA. This is in contrast to the lack of activity on such substrates by these enzymes under conventional assay conditions. In addition, the previously established activity of T4 DNA ligase on blunt-end...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)80077-9